GABAB R1 (Gamma-Aminobutyric Acid Type B Receptor Subunit 1, GABBR1, Gb1, GPRC3A)

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£761.00

SKU:: 167652
Anwendung:: IHC-Fr
Reinheit:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Gly17-Leu586 from rat GABAB R1 expressed in CHO cells.
Klon:: 751216
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Weitere Details:: GABAB R1 (GABA-B receptor subunit 1; also GABA-BR1, GABBR1 and GB1) is a multispan glycoprotein in the GABA-B receptor subfamily, GPCR-3 family of proteins. It forms an obligatory heterodimer with GABA-BR2, creating a G-protein metabotropic GABA receptor that inhibits adenylyl cyclase activity and activates K+ channels. Presynaptically, this blocks neurotransmitter release; postsynaptically, it lowers neuron excitability. Rat GABAB R1 is 991 amino acids (aa) in length. It contains a 16aa signal sequence, an extended N‑terminal extracellular region (aa17-590) that contains two SUSHI domains (aa29‑158), and a long C-terminal cytoplasmic domain (aa 885-991). There are several splice variants with predicted molecular weights ranging from 90-111kD and multiple glycosylation sites. The 991aa isoform described above is called GABAB R1e (R1e). There is also a 960aa, 130kD isoform that shows a deletion of aa771‑801. This variant (R1a) is associated with postsynaptic membranes. A third isoform (R1b) is 844aa in length and 100kD in size and possesses both a deletion of aa771-801 and a 47aa substitution for aa1-163. This variant is presynaptic in location. Two other isoforms are variants of GABAB R1b. Each show the same N‑terminal substitution, with a fourth isoform (R1c) retaining aa771-801 and a fifth isoform (R1d) deleting aa771-801, coupled to a 25aa substitution for aa935‑991. Over aa17-586, rat GABAB R1e/a shares 99% aa identity with both mouse and human GABAB R1. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml in perfusion fixed frozen sections of rat trigeminal ganglia Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice