167593
Hsp47 (SerpinH1, Colligin, Gp46, serine proteinase inhibitor, cysteine proteinase inhibitor, collagen binding protein)
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£524.00
- SKU:
- 167593
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human Hsp47, full length
- Klon:
- 1C4-1A6
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glyercol.
- Weitere Details:
- Hsp47 is a chaperone protein, member of the superfamily of serine proteinase inhibitors. Also known as SERPINH1, a serine proteinase inhibitor. It is a stress protein that resides in the endoplasmic reticulum, has an active role on the intracellular process of folding, assembly and secretion of pro-collagens. Recent studies have shown the association of on an increased expression of Hsp47 around fibrotic lesions. The identification of a novel biomarker on cell therapies aimed to reduce the progression of fibrotic diseases, could be used potentially as a universal marker, since Hsp47 binds a single substrate. Type I collagen is fundamental during the healing process after a myocardial infarction. It is critical in the position of collagen-produced cells and the assembly of collagen fibrils. Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000, 1ug/ml was sufficient for detection of Hsp47 in 20ug of heat shocked HeLa cell lysate by Western Blot using Goat anti -mouse IgG:HRP as the secondary antibody. Immunofluorescence (IC): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice