157127
Transforming Growth Factor Beta 1 (TGFb1) Recombinant, Rat
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£358.00
- SKU:
- 157127
- Molekulargewicht:
- 14.1
- Reinheit:
- ≥90%
- Lagerbedingungen:
- -70°C
- Hersteller:
- United States Biological
- Format:
- Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride pH 8.0, 0.01% sarcosyl, 5% Trehalose. Reconstitute with sterile 20mM Tris, 150mM sodium chloride pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
- Weitere Details:
- Recombinant protein corresponding to Ala279-Ser390 of rat TGFb1 with a N-terminal His-Tag expressed in E. coli. Amino Acid Sequence: AL DTNYCFSSTE KNCCVRQLYI DFRKDLGWKW IHEPKGYHAN FCLGPCPYIW SLDTQYSKVL ALYNQHNPGA SASPCCVPQA LEPLPIVYYV GRKPKVEQLS NMIVRSCKCS Predicted Isoelectric Point: 8.4 Predicted Molecular Weight: 14.1kD Accurate Molecular Mass: 16kD as determined by SDS-PAGE reducing conditions Subcellular Location: Secreted, Extracellular matrix Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Versandbedingungen:
- Blue Ice