154138
Complement Component 3a (C3a) Recombinant, Rat
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£384.00
- SKU:
- 154138
- Molekulargewicht:
- 40.3
- Reinheit:
- ≥90%
- Lagerbedingungen:
- -70°C
- Hersteller:
- United States Biological
- Format:
- Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
- Weitere Details:
- Recombinant protein corresponding to aa671-748 with an N-Terminal His and GST Tag from rat C3a, expressed in E. coli. Uniprot/Accession: P01026 Predicted Isoelectric Point: 5.4 Predicted Molecular Weight: 40.3kD Accurate Molecular Weight: 35kD and 39kD as determined by SDS-PAGE reducing conditions Subcellular Location: Secreted Amino Acid Sequence: SVQLMERRMD KAGQYTDKGL RKCCEDGMRD IPMPYSCQRR ARLITQGESC LKAFMDCCNY ITKLREQHRR DHVLGLAR Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Versandbedingungen:
- Blue Ice