Pleckstrin (FLJ27168, P47, Platelet and Leukocyte C Kinase Substrate, Platelet P47, Platelet p47 Protein, PLEK, Platelet 47kD Protein)

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£709.00

SKU:: 150826
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to 14aa, C-EWIKAIQMASRTGK, from near the C-terminus of human Pleckstrin.
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Weitere Details:: Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: ELISA: 1:16,000 Western Blot: 0.05-1ug/ml Human heart and PBMC lysates ~45kD Immunohistochemistry (paraffin): 3.75ug/ml. Human vessel (platelets) and spleen Immunofluorescence: 10ug/ml Flow Cytometry: 10ug/ml for analysis of HeLa cells Optimal dilutions to be determined by the researcher. Positive Control: Human peripheral blood leukocyte lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.