GAPDH (Glyceraldehyde-3-phosphate Dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, CDABP0047, OK/SW-cl.12, G3PD)

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£709.00

SKU:: 150044
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-HQVVSSDFNSDT, from the C-terminus of human GAPDH. Species Sequence Homology: canine and porcine
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Weitere Details:: GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blot. Applications: Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:128,000 Western Blot: 0.001-0.003ug/ml Immunohistochemistry (Paraffin): 2.5ug/ml. Heat induced antigen retrieval in citrate buffer, pH 6.0 is required Immunofluorescence: 10ug/ml Optimal dilutions to be determined by the researcher. Positive Control: 293 Cell Lysate and Human Tonsil Tissue Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.