VCAM-1, Recombinant, Human (CD106 Antigen, INCAM 100, INCAM-100, L1CAM, Vascular Cell Adhesion Molecule 1, VCAM 1, VCAM1, Vascular Cell Adhesion Protein 1)

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£451.00

SKU:: 149759
Molekulargewicht:: 74.1
Reinheit:: ≥98% (SDS-PAGE and HPLC). Endotoxin: ≤0.1ng/ug (1EU/ug)
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.0. No preservative added. Reconstitute with sterile ddH2O to 0.1-1mg/ml. Do not vortex
Weitere Details:: VCAM is a 110kD cell surface integral membrane glycoprotein that belongs to the Ig-related superfamily of adhesion molecules. The primary function of VCAM-1 is the mediation of leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 may play a vital role in the development several diseases, including atherosclerosis and rheumatoid arthritis. The human VCAM-1 gene codes for a 715aa transmembrane glycoprotein containing a 19aa cytoplasmic domain, a 22aa transmembrane domain, and a 674aa extracellular domain. Recombinant human VCAM-1 is a kD glycoprotein comprising the extracellular domain (674aa) of VCAM-1. Monomeric glycosylated VCAM-1 migrates at an apparent molecular weight of approximately 74.1kD by SDS-PAGE analysis under reducing conditions. Recombinant protein corresponding to human VCAM-1, expressed in HEK293 cells. Swiss Prot: P19320 Molecular Weight: ~74.1kD Biological Activity: Determined by its ability to support the adhesion of human U937 cells. The expected ED50 for this effect is 0.8-1ug/ml. Amino Acid Sequence: FKIETTPESR YLAQIGDSVS LTCSTTGCES PFFSWRTQID SPLNGKVTNE GTTSTLTMNP VSFGNEHSYL CTATCESRKL EKGIQVEIYSF PKDPEIHLSG PLEAGKPITV KCSVADVYPF DRLEIDLLKG DHLMKSQEFL EDADRKSLET KSLEVTFTPV IEDIGKVLVC RAKLHIDEMD SVPTVRQAVK ELQVYISPKN TVISVNPSTK LQEGGSVTMT CSSEGLPAPE IFWSKKLDNG NLQHLSGNAT LTLIAMRMED SGIYVCEGVN LIGKNRKEVE LIVQEKPFTV EISPGPRIAA QIGDSVMLTC SVMGCESPSF SWRTQIDSPL SGKVRSEGTN STLTLSPVS FENEHSYLCT VTCGHKKLEK GIQVELYSFPR DPEIEMSGGLV NGSSVTVSCK VPSVYPLDRLE IELLKGETILE NIEFLEDTDM KSLENKSLEMT FIPTIEDTGKA LVCQAKLHID DMEFEPKQRQ STQTLYVNVA PRDTTVLVSP SSILEEGSSV NMTCLSQGFP APKILWSRQL PNGELQPLSE NATLTLISTK MEDSGVYLCE GINQAGRSRK EVELIIQVTP KDIKLTAFPS ESVKEGDTVI ISCTCGNVPE TWIILKKKAE TGDTVLKSID GAYTIRKAQL KDAGVYECES KNKVGSQLRS LTLDVQGREN NKDYFSP Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice