149730
Fibroblast Growth Factor, Acidic, Recombinant, Mouse (Acidic Fibroblast Growth Factor, aFGF, FGF-alpha, FGFA, Beta-endothelial Cell Growth Factor, ECGF-beta, ECGFB, ECGF, ECGFA, FGF1, GLIO703, Heparin-binding Growth Factor 1, HBGF1, HBGF-1)
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£364.00
- SKU:
- 149730
- Molekulargewicht:
- 15.9
- Reinheit:
- ≥95% (SDS-PAGE and HPLC). Endotoxin: ≤0.1ng/ug (<1EU/ug)
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Format:
- Supplied as a lyophilized powder from 5mM sodium phosphate, pH 7.2, 100mM sodium chloride. Reconstitute in sterile 5mM sodium phosphate, pH 7.0-7.2 to 0.5-1mg/ml.
- Weitere Details:
- FGF1 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Recombinant protein corresponding to mouse FGF-acidic, expressed in E. oli. Swiss/Uniprot: P61148 Molecular Weight: ~15.9kD Biological Activity: Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 0.2ng/ml in the presence of 10ug/ml heparin, corresponding to a specific activity of ≥ 5x10e6 units/mg. Amino Acid Sequence: MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice