TRAIL, Recombinant, Mouse (TNFSF10, Tumor Necrosis Factor Ligand Superfamily Member 10, TNF-related Apoptosis-inducing Ligand, Protein TRAIL, CD253, Trail)

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£351.00

SKU:: 149712
Molekulargewicht:: 20
Reinheit:: ≥95% (SDS-PAGE gel and HPLC). Endotoxin: ≤0.1ng/ug (≤1EU/mg)
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a lyophilized powder in 10mM sodium phosphate, pH 7.0. Reconstitute with sterile ddH2O to a concentration of 0.5-1mg/ml. Do not Vortex.
Weitere Details:: TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Receptors for TRAIL include two death domain containing receptors, death receptor 4 (DR4) and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. TRAIL is a type II membrane protein and expressed in a variety of human tissues. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells. Recombinant protein corresponding to 174aa from the TNF-homologous portion of the extracellular domain of the full length mouse TRAIL, expressed in E.coli. Swiss/Uniprot: P50592 Molecular Weight: ~20kD Biological Activity: Assay 1: Determined by the dose-dependent stimulation of MIP-2 production by mouse spleen cells using a concentration range of 10-100ng/ml. Assay 2: Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells). The expected ED50 for this effect is 40-60ng/ml. Amino Acid Sequence: MRGGRPQKVA AHITGITRRS NSALIPISKD GKTLGQKIES WESSRKGHSF LNHVLFRNGE LVIEQEGLYY IYSQTYFRFQ EAEDASKMVS KDKVRTKQLV QYIYKYTSYP DPIVLMKSAR NSCWSRDAEY GLYSIYQGGL FELKKNDRIF VSVTNEHLMD LDQEASFFGA FLIN Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice