146105
BCL2A1, CT (Bcl-2-related Protein A1, Bcl-2-like Protein 5, Bcl2-L-5, Hemopoietic-specific Early Response Protein, Protein BFL-1, Protein GRS, BCL2L5, BFL1, GRS, HBPA1)
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£655.00
- SKU:
- 146105
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 14aa from near the C-terminus of human Bfl-1. Species Sequence Homology: bovine. Epitope: located within the last 50aa
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Weitere Details:
- Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumor, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells. At least two isoforms of Bfl-1 are known to exist. Presumably due to post-translational modifications, Bfl-1 is often observed at higher molecular weight in SDS-PAGE than its predicted molecular weight would suggest. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml Immunofluorescence: 20ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human Kidney Tissue Lysate or Mouse Kidney Tissue Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice