UBE3A, Recombinant, Human, 6-His tag, (ANCR, E6AP, EPVE6AP, HPVE6A, Human Papillomavirus E6-associated Protein, E6-associated Protein)

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£825.00

SKU:: 145855
Molekulargewicht:: 102
Reinheit:: ~95% (SDS-PAGE)
Lagerbedingungen:: -70°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 50mM HEPES, pH 8.0, 100mM NaCl, 1mM TCEP. BSA free.
Weitere Details:: E6AP (E6-Associated Protein) is a HECT domain ubiquitin E3 ligase that ubiquitinates and mediates the proteasomal destruction of substrate proteins. HECT domain ligases use an active site cysteine to accept charged ubiquitin from ubiquitin-E2 thioester complexes for subsequent transfer to substrate proteins; in this way HECT class ligases are distinct from most RING class ligases in that the latter facilitate transfer of ubiquitin from charged E2’s directly to substrate proteins without an E3-ubiquitin thioester intermediate. The viral HPV-E6 protein (present in cells infected with human papillomavirus) forms a complex with E6AP to generate a ligase activity that polyubiquitinates the tumor suppressors p53 and p73 and targets them to the 26S proteasome for degradation. This protein contains a C-terminal 6-His tag. Recombinant protein corresponding to Met1 - Leu875 from human His6-E6AP/UBE3A, CF. Molecular Weight: ~102kD Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.