145782
CES1, Recombinant, Mouse, aa19-565, His-Tag (Carboxylesterase 1, ACAT, Egasyn, HMSE1, REH, SES1, TGH)
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£1,017.00
- SKU:
- 145782
- Molekulargewicht:
- 5560
- Reinheit:
- ≥90% (SDS-PAGE under reducing conditions). Endotoxin: ≤0.01EU/ug (LAL).
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Format:
- Supplied as a liquid in Tris, NaCl. BSA free.
- Weitere Details:
- Carboxylesterase 1 is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds (1, 2). They have broad substrate specificity ranging from small molecule esters such as phenylester to long chain fatty acid esters and thioesters. They play a major role as determinants of pharmacokinetic behavior for most therapeutic agents containing an ester. By deesterification, they can activate or inactivate the agents. They also participate in detoxification of drugs such as cocaine and heroin in serum and liver. The resulting deesterified metabolites are secreted out in urine. They can also detoxify organophosphate and carbamate analogues used in agrochemicals or chemical nerve agents, such as malathion, sarin, tabun, and VX. In addition to the hydrolytic activity, they can perform transesterification, a reaction important for cholesterol homeostasis. Carboxylesterase deficiency may be associated with nonHodgkin lymphoma or Bcell lymphocytic leukemia. CES1 shares the serine hydrolase fold observed in other esterases (3). Ces1G is a rat and mouse specific protein that is expressed predominantly in liver, but also in kidney and lung (4). Recombinant protein corresponding to His19-Leu565 from mouse Carboxylesterase 1/CES1, fused to His-tag at C-terminal, expressed in NS0 cells. Molecular Weight: ~55-60kD Biological Activity: Measured by its ability to hydrolyze p-nitrophenylacetate. The specific activity is >330 pmol/min/μg Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice