145647
Ubiquilin 1 (DA41, DSK2, PLIC-1, UBQLN1, UBQN, XDRP1, PLIC-1, Protein Linking IAP with Cytoskeleton 1)
Größe
£843.00
- SKU:
- 145647
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Arg546-Gly585 from human Ubiquilin 1, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Weitere Details:
- Ubiquilin-1 (also known as DA41 and PLIC-1/Protein Linking IAP with Cytoskeleton 1) is a 62-70 kDa intracellular member of the UBL-UBA family of proteins. Consistent with its name, it ubiquitously expressed, and is particularly prominent in skeletal muscle and neurons. Multiple functions have been attributed to ubiquilin-1. For instance, it is now known to be a key player in APP processing. On the cleavage side, ubiquilin-1 binds to PS/presenilin, a component of the gamma -secretase complex that cleaves APP. Ubiquilin-1 appears to target both ubiquitinated single chain PS to the proteosome, and aggregated PS to the aggresome. With respect to APP, ubiquilin-1 serves as a molecular chaperone, binding to and inhibiting APP aggregation. On cells expressing CD47, Ubiquilin-1 acts as an intermediary between integrins, the cytoskeleton and CD47, promoting cell spreading. And in neurons, ubiquilin-1 stabilizes both membrane and GABA subunit stability, enhancing inhibitory synaptic transmission. Human ubiquilin-1 is 589 amino acids (aa) in length. It contains one ubiquitin-like domain (aa 37-111), an S5a binding site (aa 72-105) and a UBA binding region (aa 548-585). There are multiple splice variants, ranging from 19-59 kDa in size. One shows a deletion of aa 418-445, a second contains a three aa substitution for aa 151-589, a third possesses a 29 aa substitution for aa 112-589, and a fourth shows a deletion of aa 61-237 coupled to a second deletion of aa 361‑589. Over aa 546-585, human and mouse ubiquilin-1 are identical in amino acid sequence. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml with TT human medullary thyroid cancer cell line, IMR‑32 human neuroblastoma cell line, and PC‑3 human prostate cancer cell line under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice