145646
TXNL2 (Glutaredoxin 3, GLRX3, GLRX4, GRX3, GRX4, PICOT, PKC-theta-interacting Protein, Thioredoxin-like protein 2, TXNL)
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£800.00
- SKU:
- 145646
- Reinheit:
- Purified by Protein A or G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa126-294 from human Glutaredoxin 3/GLRX3, expressed in E. coli (UniProt # O76003).
- Klon:
- 791431
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- GLRX3 (Glutaredoxin 3; also PICOT, PKCthetainteracting protein, and Thioredoxinlike protein 2/TXNL2) is a 3740kD member of the multidomain subgroup, monothiol glutaredoxin group of glutaredoxin/GTX proteins. It has widespread expression but is not found ubiquitously. Cells known to express cytosolic GLRX3 are principally epithelial in type, and include breast and adrenal epithelium, pancreatic exocrine gland epithelium, and proximal tubule renal epithelium. Cardiac muscle may also express GLRX3. By inference, it is suggested to be involved in cell proliferation and has also been associated with both PKCtheta regulation and MLP/muscle LIM proteinmediated cardiac contractility. Human GLRX3 is 335 amino acids (aa) in length. It contains an Nterminal thioredoxinlike domain (aa2117) that is followed by two PICOT homology domains (aa144236 and 237335). The thioredoxin domain does not possess a typical disulfide, and it is suggested that this domain does not demonstrate redox activity. The PICOT domains will interact with intracellular proteins. GLRX3 exists as both a monomer and homodimer, with the homodimer incorporating two Fe/S clusters into their PICOT domains. These clusters serve as redox sensors within the cell. Over aa126294, human GLRX3 shares 98% aa sequence identity with mouse GLRX3. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice