145619
Taxilin, alpha (TXLNA) TXLN-alpha, IL14alpha)
Größe
£761.00
- SKU:
- 145619
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met1-Lys162 from human Taxilin, alpha expressed in E. coli.
- Klon:
- 812901
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute in 200ul sterile PBS.
- Weitere Details:
- alpha ‑Taxilin (TXLN‑ alpha ; also p75 [in rat] and IL‑14 alpha ) is the founding member of the taxilin family of proteins. Although its predicted MW is 62 kDa, it runs anomalously at 74‑76 kD in SDS‑PAGE. alpha ‑Taxilin is ubiquitously expressed and participates in intracellular vesicle trafficking. In particular, alpha ‑Taxilin interacts with syntaxin family members that localize to the plasma membrane, providing for SNARE complex formation. alpha ‑Taxilin also regulates the placement of growing protein chains. Human alpha ‑Taxilin is 546 amino acids (aa) in length. Its defining characteristic is a large coiled‑coil region between aa 186‑491. When an alternate start site at Met176 is used, a secreted cytokine is generated termed IL‑14 alpha. Over aa 1‑162, human alpha ‑Taxilin shares 81% aa identity with mouse alpha ‑Taxilin. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml HepG2 human hepatocellular carcinoma cell line and HEK293 human embryonic kidney cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice