145586
PSMA2 (Proteasome Subunit alpha Type 2, Proteasome Component C3 and Macropain Subunit C3, HC3, MU, PMSA2, PSC3)
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£825.00
- SKU:
- 145586
- Anwendung:
- WB
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to aa2-234 from human PSMA2, expressed in E. coli (UniProt # P25787).
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Weitere Details:
- PSMA2 (Proteasome subunit alpha type 2; also proteasome component C3 and Macropain subunit C3) is an intracellular 25-27kD member of the peptidase T1A family of molecules. It is ubiquitously expressed, and participates in the formation of the 700kD 20S proteasomal complex. This complex contains four stacked rings, forming a barrel. The top and bottom rings are made of seven alpha-subunits, while the two central rings are composed of seven catalytic beta-subunits. The complex degrades 80-90% of all intracellular proteins through both a ubiquitin-dependent and -independent process. PSMA2 may modulate the activity of select beta-subunits. Human PSMA2 is 234 amino acids (aa) in length. It contains at least five utilized phosphorylation sites plus one nitrated tyrosine. Full-length human PSMA2 shares 99% aa sequence identity with mouse PSMA2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice