LRIG3 (LIG-3 (Leucine-rich Repeats and Ig-like Domains-3)

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£806.00

SKU:: 145510
Reinheit:: Purified by Protein A affinity chromatography from hybridoma culture supernatant
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Asp28-Thr807 from human LRIG3, expressed in NS0 cells
Klon:: 814834
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Weitere Details:: LRIG3 (leucine-rich repeats and Ig-like domains-3) is an ~140-170kD type I transmembrane glycoprotein member of the mammalian LRIG glycoprotein family. This family contains three members who share 45-50% amino acid (aa) identity. All members contain fifteen LRRs, accompanied by two flanking cysteine-rich regions, and three C2-type-Ig-like domains in their extracellular domains (ECD). LRIG3 mRNA is widely expressed, with highest levels in stomach, skin, thyroid and small intestine. Human LRIG3 is synthesized as a 1120 amino acid (aa) precursor. It contains a 24aa signal sequence, a 786aa ECD, a 21aa transmembrane sequence, and a 289aa intracellular region. One splice variant exists that has a 19aa substitution for the first 79aa of the standard (or long) form. This substitution appears to encode an alternate signal sequence, resulting in a mature protein that lacks the first and part of the second LRR. LRIG1, a related family member, is known to bind the EGF family receptors ErbB1-4, via either its LRR or Ig-like domains. It also binds the ubiquitin ligase, c-Cbl, and promotes ubiquitination, internalization and destruction of these receptors. It is not known whether LRIG3 performs similar functions. Within the cell, LRIG3 is expressed in the perinuclear region as well as on the cell surface. Perinuclear location of LRIG3 in grade III and IV astrocytic tumors has been associated with better patient survival. Human LRIG3 ECD shares 91%, 92%, 95% and 98% aa sequence identity with mouse, rat, bovine and canine LRIG3 ECD, respectively. Applications: Suitable for use in Flow Cytometry, ELISA, and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells. Using KATO-III human gastric carcinoma cell line Western Blot: 1ug/ml using KATO-III human gastric carcinoma cell line, HEK293T human embryonic kidney cell line and B16-F1 mouse melanoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice