145452
Endophilin B1 (Bif-1, Endophilin B1/B2, SH3-domain GRB2-like Endophilin B1/Bax-interacting Factor 1, SH3-domain GRB2-like Endophilin B1, SH3GLB1)
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£761.00
- SKU:
- 145452
- Reinheit:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ala33-Asn189 from human Endophilin B1/Bif‑1, expressed in E. coli.
- Klon:
- 807009
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- Endophilins are among the best known BAR (Bin/Amphiphysin/Rvs) domain proteins. The highly conserved protein dimerisation domain is typically involved in membrane dynamics. Endophilin-B1, also known as SH3GLB1 (Src-Homology 3 Domain-containing GRB2-like protein B1) and BIF-1, is a 40 kDa protein that belongs to the endophilin family of molecules. It is a cytoplasmic and Golgi membrane protein that is expressed in neurons, striated (skeletal and cardiac) muscle cells, and placenta. Using SH3GLB1 in yeast two-hybrid screens, a second protein, SH3GLB2 (endophilin B2), was identified as an interacting partner. Endophilin-B1 plays a role in cell homeostasis. In neurons, Endophilin-B1 is phosphorylated by Cdk5, inducing homodimerization and autophagosome formation. In addition, it appears to play a role in the maintenance of mitochondrial integrity. Endophilin-B1 appears to cycle on-and-off the outer mitochondrial membrane (OMM), contributing to OMM integrity. And after the initiation of endocytosis, it also directs EEA1+ TrkA-containing endosomes back into the cell membrane for reuse. Human Endophilin-B1 is 365 amino acids (aa) in length. It possesses an N-terminal lipid-binding BAR (Bin/Amphiphysin/Rvs) domain (aa 27-261) that contains an internal coiled-coil region, and a C‑terminal SH3 domain that binds Pro-rich sequences on potential ligands (309-363). There are at least three potential splice variants for Endophilin-B1. Two are 42 and 43 kDa in size, and possess a 21 and 29 aa insertion after Ser190, respectively. A third potentially utilizes an alternative start site at Met101. Over aa 33-189, human Endophilin-B1 shares 98% aa sequence identity with mouse Endophilin-B1. Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml using jurkat human acute T cell leukemia cell line, Saos‑2 human osteosarcoma cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line as a sample. Immunohistochemistry: 8-25ug/ml in immersion fixed paraffin-embedded sections of human pancreas. Immunocytochemistry: 5-25ug/ml in immersion fixed Jurkat human acute T cell leukemia cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice