144939
Myoglobin (MB)
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£620.00
- SKU:
- 144939
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A synthetic peptide corresponding to a sequence at the C-terminal of mouse Myoglobin, identical to the related rat sequence.
- Format:
- Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitutie with 200ul sterile ddH2O.
- Weitere Details:
- Myoglobin(MB) also known as PVALB, is a single-chain globular protein of 153 or 154 amino acids, containing a heme (iron-containing porphyrin)prosthetic group in the center around which the remaining apoprotein folds. Two structural variants of myoglobin were described by Boyer et al. (1963). Boulton et al. (1969) studied postmortem muscle from 2,500 persons. Twomyoglobin variants were found, and in one of these substitution of lysine for glutamic acid at residue 53 was demonstrated. Myoglobin was the first protein to have its three-dimensional structure revealed. Jeffreys et al. (1984) used DNA probes isolated from the cloned myoglobin gene to map the gene in human-rodent somatic cell hybrids. The myoglobinlocus mapped to chromosome 22q11-q13. Myoglobin is released from damaged muscle tissue (rhabdomyolysis), which has very high concentrations of myoglobin. The released myoglobin is filtered by the kidneys but is toxic to the renal tubular epithelium and so may cause acute renal failure. UniProt Number: P04247 Gene ID: MB Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: paraffin Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice