143693-PE
VEGF (Vascular Endothelial Growth Factor, Vascular Endothelial Growth Factor A, VEGFA, VEGF-A, MGC70609, MVCD1, Vascular Permeability Factor, VPF) (PE)
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£722.00
- SKU:
- 143693-PE
- Reinheit:
- Purified by Protein A affinity chromatography from cell culture.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human VEGF165 expressed in E. coli.
- Klon:
- 14G22
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
- Weitere Details:
- Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Applications: Suitable for use in FLISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilution: FLISA: In a sandwich FLISA (assuming 100ul/well), a concentration of 2-4ug/ml of this antibody will detect at least 100pg/ml of recombinant human VEGF165 when used with biotinylated antigen affinity purified anti-human VEGF165 as the detection antibody at a concentration of approximately 0.25-1ug/ml. Western Blot: To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.5ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.5ng/lane, under non-reducing conditions. Neutralization: To yield one-half maximal inhibition of the biological activity of Human VEGF (10ng/ml), a concentration of 0.2ug/ml of this antibody is required. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice