143693-BIOTIN
VEGF (Vascular Endothelial Growth Factor, Vascular Endothelial Growth Factor A, VEGFA, VEGF-A, MGC70609, MVCD1, Vascular Permeability Factor, VPF) (Biotin)
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£722.00
- SKU:
- 143693-BIOTIN
- Reinheit:
- Purified by Protein A affinity chromatography from cell culture.
- Lagerbedingungen:
- 4°C/-20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human VEGF165 expressed in E. coli.
- Klon:
- 14G22
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
- Weitere Details:
- Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilution: ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 2-4ug/ml of this antibody will detect at least 100pg/ml of recombinant human VEGF165 when used with biotinylated antigen affinity purified anti-human VEGF165 as the detection antibody at a concentration of approximately 0.25-1ug/ml. Western Blot: To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.5ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.5ng/lane, under non-reducing conditions. Neutralization: To yield one-half maximal inhibition of the biological activity of Human VEGF (10ng/ml), a concentration of 0.2ug/ml of this antibody is required. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice