Il10 (Interleukin-10, IL-10, Interleukin 10, Cytokine Synthesis Inhibitory Factor, CSIF)

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£479.00

SKU:: 143596
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rat
Immunogen:: Recombinant mouse IL-10
Klon:: 14G9
Format:: Supplied as a lyophilized powder. Reconstitute with sterile ddH2O.
Weitere Details:: The protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilution: ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 4-8ug/ml of this antibody will detect at least 1600pg/ml of recombinant murine IL-10 when used with biotinylated antigen affinity purified anti-murine IL-10 as the detection antibody at a concentration of approximately 0.5-1ug/ml. Western Blot: To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1-2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2-4ng/lane, under reducing or non-reducing conditions. Neutralization: To yield one-half maximal inhibition of the biological activity of Murine IL-10 (0.5ng/ml), a concentration of 3-5ug/ml of this antibody is required. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice