Egf (Pro-epidermal Growth Factor, EGF, Epidermal Growth Factor) (Biotin)

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£479.00

SKU:: 143558
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to rat EGF, expressed in E. coli
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with of sterile PBS containing 01% BSA. Labeled with Biotin.
Weitere Details:: Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (sandwich): 0.25-1ug/ml using 100ul/well antibody solution, in conjunction with E3374-05W as a capture antibody, allows the detection of 0.2-0.4ng/well of recombinant rat EGF. Western Blot: 0.5-1ug/ml; The detection limit is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS containing 01% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.