MIF, Recombinant, Human (Macrophage Migration, Inhibitory Factor, GLIF, MMIF, GIF, Glycosylation-inhibiting Factor)

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£320.00

SKU:: 143435
Molekulargewicht:: 15
Reinheit:: ≥98% (HPLC, SDS-PAGE). Endotoxin: ≤0.1ng/ug (1EU/ug)
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a lyophilized powder from 10mM PBS, pH 7.5. Reconstitute sterile ddH2O at a concentration of 0.1-1mg/ml.
Weitere Details:: Macrophage migration inhibitory factor (MIF) is a small secreted protein that can act as a pleiotropic pro-inflammatory cytokine, as well as an enzyme. MIF pro-inflammatory activity can be initiated by signaling through CD74 and CD44, resulting in the secretion of TNF-a, IL-1, IL-6, IL-8, and various MMPs. The enzymatic activity of MIF is characterized by its ability to act as a tautomerase, capable of catalyzing the keto-to-enol isomerization of keto-phenylpyruvate and L-dopachrome. It appears as though MIF catalytic activity is dependent upon a trimeric configuration and a free N-terminal proline residue. Insect cell-derived recombinant human MIF is a 15kD protein containing 124 amino acid residues, including an N-terminal His-tag. Biological Activity: Determined by its ability to inhibit monocyte migration. Amino Acid Sequence: HHHHHHHHAM PMFIVNTNVP RASVPDGFLS ELTQQLAQAT GKPPQYIAVH VVPDQLMAFG GSSEPCALCS LHSIGKIGGA QNRSYSKLLC GLLAERLRIS PDRVYINYYD MNAANVGWNN STFA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice