Pepsin (PP)

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£482.00

SKU:: 141789
Reinheit:: Purified by oaffinity chromatography
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Native pepsin protein
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol
Weitere Details:: Pepsin is an enzyme whose zymogen (pepsinogen) is released by the chief cells in the stomach and that degrades food proteins into peptides. Pepsin is expressed as a pro-form zymogen, pepsinogen, whose primary structure has an additional 44 amino acids. Pepsin is most active in acidic environments between 37ºC and 42ºC. Accordingly, its primary site of synthesis and activity is in the stomach (pH 1.5 to 2). Pepsin exhibits maximal activity at pH 2.0 and is inactive at pH 6.5 and above, however pepsin is not fully denatured or irreversibly inactivated until pH 8.0. Pepsin is used for a variety of applications in food manufacturing and is commonly used in the preparation of F(ab')2 fragments from antibodies. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry AND Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:100-1:200 Western Blot: 1:100-1:400 Immunohistochemistry (frozen): 1:100-1:500 Immunohistochemistry (Paraffin): 1:50-1:200 Immunocytochemistry: 1:100-1:500 Optimal dilutions to be determined by the researcher. Included Protein Molecular Weight Marker: 168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed. MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice