Complement Component 5a (C5a)

Größe

£468.00

SKU:: 139875
Reinheit:: Purified by affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa679-755 with an N-Terminal His-Tag from mouse C5a, expressed in E. coli
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Weitere Details:: Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:100-1:200 Western Blot: 1:50-1:400 Immunohistochemistry (frozen): 1:50-1:500 Immunohistochemistry (Paraffin): 1:10-1:100 Immunocytochemistry: 1:50-1:500 Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MGSSHHHHHH-SSGLVPRGSHMASMTGGQQMGRGS-NL HLLRQKIEEQ AAKYKHSVPK KCCYDGARVN FYETCEERVA RVTIGPLCIR AFNECCTIAN KIRKESPHKP VQLGR Positive Control (Supplied): 139875A: Suppled as a recombinant protein (aa679-755) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.