138531-AF-ML650
Chorionic Gonadotropin, Human, beta (hCG beta) (MaxLight 650)
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£876.00
- SKU:
- 138531-AF-ML650
- Reinheit:
- Purified by Protein A/G affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full-length recombinant protein corresponding to human hCG-beta. Cellular Localization: Cytoplasmic, secreted
- Klon:
- HCGb/54
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Weitere Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. This monoclonal antibody reacts with a protein of 22kD, identified as bet a sub-unit of HCG. It does not cross react with the alpha sub-unit. HCG is a glycoprotein, which is secrete d in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide cha ins designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stim ulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). hCG monoclonal antibody detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarc inoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of l ung squamous cell carcinomas are positive. hCG expression by non-trophoblastic tumors may indicate aggressive behavior. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher. Positive Control: JAR or TT cells, Placenta Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice