CD3e (CD 3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex)

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£646.00

SKU:: 138514-AF
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa156-168 of human CD3e (ERPPPVPNPDYEP). Cellular Localization: Cell surface and cytoplamic.
Format:: Supplied as a liquid in PBS. Also available with BSA and azide, see 138514.
Weitere Details:: The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml for 60 minutes at RT Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cells. Tonsil and lymph node Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice