138512
CD34 (Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin) (PE)
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£646.00
- SKU:
- 138512
- Reinheit:
- Purified by Protein A/G affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Blast cells of a chronic myeloid leukemia patient. Cellular Localization: Cell surface
- Klon:
- ICO-115
- Format:
- Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE).
- Weitere Details:
- This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kD, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Applications: Suitable for use in Flow Cytometry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ul/test to label 10e6cells or 5ul/100ul of whole blood Immunofluorescence: 1:50-1:100 Optimal dilutions to be determined by the researcher. Positive Control: KG-1 cells, tonsil or angiosarcoma Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Versandbedingungen:
- Blue Ice