138510-AF

CD31 (PECAM-1, EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA)

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£646.00

SKU:: 138510-AF
Reinheit:: Purified by Protein A/G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Full-length recombinant protein corresponding to human CD31. Cellular Localization: Cell junction, cell membrane, membrane raft.
Klon:: C31.7
Format:: Supplied as a liquid in PBS. Also available with BSA and azide, see 138510.
Weitere Details:: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 monoclonal antibody reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence. Applications: Suitable for use in ELISA, Immunofluorescence, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 2-4ug/ml Flow Cytometry: 1-2ug/million cells Immunofluorescence: 1-3ug/ml Immunohistochemistry (Frozen & FFPE): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris 1mM EDTA, pH 9.0, for 45 minutes at 95ºC followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cells, THP-1 cell lysate, angiosarcoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice