PDE4D2, Active, Recombinant, Human, GST-Tag (PDE4D, cAMP-specific 3',5'-cyclic Phosphodiesterase 4D, DPDE3, PDE43, DPDE3)

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£1,059.00

SKU:: 137343
Molekulargewicht:: 84
Reinheit:: ≥85%
Lagerbedingungen:: -70°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 3mM DTT, 16mM glutathione, 20% glycerol.
Weitere Details:: Cyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprise of 5 variants (PDE4D1, D2, D3, D4 and D5; 1, 2). One or more PDE4D subtype-variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype-variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short-term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanism involved protein-protein interactions with cytoskeletal scaffolding proteins. Recombinant corresponding to aa2-507 from human PDE4D2, fused to GST-tag at N-terminal, expressed in a Baculovirus infected Sf9 cell expression system. Accession Number: NM_001197221 Molecular Weight: ~84kD Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Specific Activity: ≥13,600pmol/min/ug Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Dry Ice