MAP Kinase 10, Active, Recombinant, Human, GST-Tag (Mitogen-activated Protein Kinase 10, MAPK 10, MAPK10, PRKM10, c-Jun N-terminal Kinase 3, JNK3, JNK3A, MAP Kinase p49 3F12, p493F12, p54bSAPK, Stress-activated Protein Kinase 1b, SAPK1b, Stress-activated Protein Kinase JNK3)

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£912.00

SKU:: 137311
Molekulargewicht:: 75
Reinheit:: ~90%
Lagerbedingungen:: -70°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM KCl, 0.04% Tween-20, 3mM DTT, 20% glycerol
Weitere Details:: MAPK10 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kinase 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Recombinant corresponding to aa2-422 from human MAPK10, fused to GST-tag at N-terminal, expressed in Sf9 cells via a baculovirus expression system. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Specific Activity: ~22pmol/min/ug. Enzyme reaction was performed in 50ul kinase reaction buffer (40mM Tris pH 7.4, 10mM MgCl2, 0.1mg/ml BSA, 0.5mM DTT) containing 50uM ATP, 0.2mg/ml ATF2 of (Cat #A0855-42H1) and 2-16ng/µl MAPK10 for 30 minutes at 30°C. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Dry Ice