SMPD1 (Sphingomyelin Phosphodiesterase, Acid Sphingomyelinase, aSMase, ASM)

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£685.00

SKU:: 133577
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Full length recombinant corresponding to aa1-365 from human SMPD1 (AAH41164) with GST tag. MW of the GST tag alone is 26kD.
Klon:: 4H2
Format:: Supplied as a liquid in PBS, pH 7.2.
Weitere Details:: SMPD1 (Sphingomyelin phosphodiesterase 1; also ASM) is a 70-72kD member of the sphingomyelinase family of enzymes. It is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide through the removal of phosphorylcholine. This generates second messenger components that participate in signal transduction. Mature human SMPD1 is 583aa in length. It contains one saposin B-type domain (aa85-169) that likely interacts with sphingomyelin, and a catalytic region (aa 267-363). There are multiple isoforms. One is 57kD in size and results from proteolytic cleavage between Pro179-Pro197. Three others show aa substitutions; a 56aa substitution for (aa1-361), a two aa substitution for (aa363-629), a12aa substitution for (aa363-418). Over (aa62-628), human SMPD1 is 86% aa identical to mouse SMPD1. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MPRYGASLRQSCPRSGREQGQDGTAGAPGLLWMGLALALALALALALSDSRVLWAPAEAHPLSPQGHPARLHRIVPRLRDVFGWGNLTCPICKGLFTAINLGLKKEPNVARVGSVAIKLCNLLKIAPPAVCRSIVHLFEDDMVEVWRRSVLSPSEACGLLLGSTCGHWDIFSSWNISLPTVPKPPPKPPSPPAPGAPVSRILFLTDLHWDHDYLEGTDPDCADPLCCRRGSGLPPASRPGAGYWGEYSKCDLPLRTLESLLSGLGPAGPFDMVYWTGDIPAHDVWHQTRQDQLRALTTVTALVRKFLGPVPVYPAVGNHESTPVNSFPPPFIEGNHSSRWLYEAMAKAWEPWLPAEALRTLRCI Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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