131852
Progesterone Receptor (PgR, PR, Nuclear Receptor Subfamily 3 Group C Member 3, NR3C3)
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£685.00
- SKU:
- 131852
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Partial recombinant protein corresponding to aa1-110 from human PGR (NP_000917) with GST tag. MW of the GST tag alone is 26kD.
- Klon:
- 3E11
- Format:
- Supplied as a liquid in PBS, pH 7.2.
- Weitere Details:
- The human progesterone receptor (hPR) belongs to the superfamily of nuclear receptors (including those for steroid and thyroid hormones, and retinoids) whose members coordinate morphogenesis and homeostasis in response to the binding of their ligands. These receptors act as transcriptional factors that regulate gene expression positively or negatively by interacting with cognate DNA sequences. It exists as two functionally distinct isoforms, PR-A and PR-B. The proteins are identical except for an additional 164 residues located at the N terminus of PR-B. whereas both isoforms appear to have similar DNA binding affinities, PR-B in fact has a greatly increased intrinsic binding affinity and cooperative binding ability relative to PR-A. The differences map to a unique AF3 in the B-upstream segment (BUS), at the far N terminus of PR-B, which is missing in PR-A. It maps to 11q22-q23 region of human chromosome. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 10ug/ml Immunohistochemistry (FFPE): 3ug/ml Optimal dilutions to be determined by the researcher. AA Sequence: MTELKAKGPRAPHVAGGPPSPEVGSPLLCRPAAGPFPGSQTSDTLPEVSAIPISLDGLLFPRPCQGQDPSDEKTQDQQSLSDVEGAYSRAEATRGAGGSSSSPPEKDSGL Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice