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130594-BIOTIN

NT5C (5'(3')-deoxyribonucleotidase, Cytosolic Type, Cytosolic 5',3'-pyrimidine Nucleotidase, Deoxy-5'-nucleotidase 1, dNT-1, DNT1, UMPH2, DNT, DNT-1, P5N2, PN-I, PN-II) (Biotin)

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£914.00

SKU:
130594-BIOTIN
Reinheit:
Purified by Protein A affinity chromatography.
Lagerbedingungen:
-20°C
Hersteller:
United States Biological
Host:
Mouse
Immunogen:
Full length recombinant corresponding to aa1-562 from NT5C2 (AAH01595) with GST tag. MW of the GST tag alone is 26kD.
Klon:
3C1
Format:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Weitere Details:
Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides, with a preference for dUMP and dTMP, intermediate activity towards dGMP, and low activity towards dCMP and dAMP. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml Immunofluorescence: 10ug/ml Optimal dilutions to be determined by the researcher. AA Sequence: MSTSWSDRLQNAADMPANMDKHALKKYRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKY Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.  For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.  Note: Applications are based on unconjugated antibody.
Versandbedingungen:
Blue Ice