130520-ML550
NQO1 (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, QR1, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase 1, DIA4, NMOR1) (MaxLight 550)
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£914.00
- SKU:
- 130520-ML550
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full length recombinant corresponding to aa1-274 from human NQO1 (AAH07659) with GST tag. MW of the GST tag alone is 26kD.
- Klon:
- 1E3-A6
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
- Weitere Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. NQO1 is a 274aa protein belonging to the NAD(P)H dehydrogenase (quinone) family. Its enzymatic activity prevents reduction of quinones that results in the production of radical species and is involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It plays a role in regulating ubiquitin-independent degradation of ornithine decarboxylase by the 20S proteasome and regulates p53 proteasomal degradation in cancer. Defects in NQO1 lead to tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, susceptibility to various forms of cancer and Alzheimer's disease (AD). It is widely expressed. Applications: Suitable for use in Immunofluorescence, FLISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunofluorescence: 10ug/ml Sandwich FLISA: The detection limit is ~0.3ng/ml as a capture antibody Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice