124997-BIOTIN
CIB1 (Calcium and Integrin-binding Protein 1, CIB, Calcium- and Integrin-binding Protein, CIBP, Calmyrin, DNA-PKcs-interacting Protein, Kinase-interacting Protein, KIP, SNK-interacting Protein 2-28, SIP2-28, CIB, KIP, PRKDCIP) (Biotin)
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£927.00
- SKU:
- 124997-BIOTIN
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full length recombinant corresponding to aa1-191 from CIB1 (AAH00846) with GST tag. MW of the GST tag alone is 26kD.
- Klon:
- 4D2-3A8
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
- Weitere Details:
- CIB1 (Calcium and Integrin Binding protein1; also calmyrin, KIP and CIBP) is a 22-23kD, Ca++ binding member of the CIB family of proteins. It is widely expressed, being found in neurons, platelets/megakaryocytes, skeletal muscle myocytes and lymphocytes. CIB1 is associated with the cytosolic side of the plasma membrane, and has multiple binding partners, including InsP3R, GPIIb aIIb, presenilin 2, and NBR1 plus FEZ. When CIB1 binds InsP3R, this ER-embedded receptor is both activated, and later desensitized to subsequent ligand binding. Relative to GPIIb, CIB1 interaction with the aIIbB 3 integrin on platelets following thrombin exposure appears to inhibit integrin activation, thus providing a tight control on subsequent platelet binding to fibrinogen. Human CIB1 is 191aa in length. It contains a utilized myristoylation site at 2aa, followed by two EF-hand domains (aa103-183). CIB1 is suggested to act as a monomer. There is at least one potential isoform variant that shows a 40aa insertion after 29aa. Full length human CIB1 (aa1-191) shares 94% aa sequence identity with mouse CIB1. Applications: Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 10ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml Optimal dilutions to be determined by the researcher. AA Sequence: MGGSGSRLSKELLAEYQDLTFLTKQEILLAHRRFCELLPQEQRSVESSLRAQVPFEQILSLPELKANPFKERICRVFSTSPAKDSLSFEDFLDLLSVFSDTATPDIKSHYAFRIFDFDDDGTLNREDLSRLVNCLTGEGEDTRLSASEMKQLIDNILEESDIDRDGTINLSEFQHVISRSPDFASSFKIVL Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice