124728-ML490
CDC2 (Cell Division Control Protein 2 Homolog, CDC28A, Cell Division Protein Kinase 1, Cyclin-dependent Kinase 1, CDK1, p34 Protein Kinase, P34CDC2) (MaxLight 490)
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£934.00
- SKU:
- 124728-ML490
- Anwendung:
- WB
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Full length human CDC2, aa1-297 (NP_001777.1).
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
- Weitere Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. CDC2/CDK1, a CDC2/CDK -type protein kinase, regulates cell cycle transitions G1 to S and G2 to M. CDC2/CDK1 is maintained in an inactive state by phosphorylation by kinases MYT1 and WEE1. The active kinase, called the M-phase promoting factor, consists of a heterodimer with cyclin B, and final activation at G2/M results from CDC25 phosphatase-mediated dephosphorylation of CDC2/CDK1. The drug Taxol has been shown to activate CDC2/CDK1 in MDA-MB-435 breast cancer cells, leading to cell cycle arrest at G2/M and to apoptosis. CDC2 has also been implicated in neurofibrillary degeneration in Alzheimer's disease and other neurodegenerative diseases. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice