059478
SFTPC (proSP-C) (Not for Export EU)
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£895.00
- SKU:
- 059478
- Reinheit:
- Serum
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- GST-tagged recombinant protein corresponding to the N-terminal region of human Prosurfactant Protein C (proSP-C).
- Format:
- Supplied as a liquid, 0.05% sodium azide.
- Weitere Details:
- Pulmonary surfactant is a complex mixture of phospholipids and proteins secreted from airway epithelial cells (type II cells) in alveoli which reduces the surface tension at the alveolar air liquid interface, providing alveolar stability necessary for normal ventilation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C and D. SP A (28-36kD) and SP D (43kD) are collagenous carbohydrate binding proteins, whereas SP B (8-9kD) and SP C (4kD) are non collagenous hydrophobic proteins. SP C is a fragment of a much larger precursor protein of 21kD. The precursor contains an exceedingly hydrophobic region of 34 amino acids that comprises most of the mature SP C, and a unique poly-valine domain. Preincubation of the antiserum with the fusion protein results in ablation of immunostaining. On human tissue immunostains alveolar Type II cells in normal lung. On mouse tissue immunostains alveolar Type II cells in normal lung. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (FFPE): 1:2000 detected Prosurfactant Protein C (proSP-C) in mouse lung tissue sections. Western Blot: 1:500 detected Prosurfactant Protein C (proSP-C) in mouse lung tissue lysate and GST-fusion Prosurfactant Protein C fragment. ELISA: 1:50,000 detected GST-fusion Prosurfactant Protein C fragment. Optimal dilutions to be determined by the researcher. Control: Mouse lung epithelial cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice