059461
Heat Shock Protein 90α
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£709.00
- SKU:
- 059461
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-conjugated linear peptide corresponding to the N-terminus of human Heat Shock Protein 90α. Species Sequence Homology: canine (100%)
- Format:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Weitere Details:
- The Heat Shock Protein 90 family is known as a group of molecular chaperones involved in the regulation of maturation, degradation, and folding transport for a select, yet varied group of proteins primarily comprised of signaling molecules, but also including tyrosine kinases, G-protein subunits, transcription factors, and telomerase. Heat Shock Protein 90 has two isoforms that share a homology of 76%; Heat Shock Protein 90α (HSP90α) and Heat Shock Protein 90β (HSP90β). HSP90α contains a charged sequence linker, a C-terminal domain, and N-terminal domain and a middle domain. It is induced by oxidative stress and has been observed in exosomes where it is secreted into the extracellular environment following translocation via TGFα stimulus. Extracellular HSP90α functions to facilitate the migration of both dermal and epidermal cells through its interaction with cell surface receptors CD91 and LRP1. HSP90α interaction with TERT is a necessary component of TERT holoenzyme complex assembly and stabilization. It has also been shown to interact with a variety of other proteins such as FNIP1, HSF1, TOM34, AHSA1, SMYD3, and DNAJC7. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml detected Heat Shock Protein 90α on 10ug of NIH/3T3 cell lysate. Immunohistochemistry (paraffin): 1:100 dilution from a previous lot detected Heat Shock Protein 90α in human prostate adenocarcinoma tissue using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunocytochemistry: 1:500 detected Heat Shock Protein 90α in A431 cells. Immunoprecipitation: 10ug immunoprecipitated Heat Shock Protein 90A from HeLa cell lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice