Vimentin (VIM)

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£780.00

SKU:: 059360
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Vimentin.
Klon:: 12E4
Format:: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Weitere Details:: Vimentin is a cytoskeleton intermediate filament protein. It is composed of monomers that each contain a central α-helix rod domain, which facilitates formation of a coiled-coil dimer required for vimentin filament assembly, as well as N-terminal head and C-terminal tail domains. It is expressed in mesenchymal stem cells and cells of mesenchymal origin, including leukocytes, endothelial cells, and smooth muscle cells. Vimentin is attached to nuclei, endoplasmic reticulum, and mitochondria, and has a role in positioning organelles in the cytosol. It regulates glial morphology, facilitates motility and directional migration of fibroblasts, and is critical to mechanotransduction of shear stress and maintenance of vascular endothelial integrity. Vimentin controls transport of LDL-derived cholesterol from lysosomes to esterification sites. It is an aggresome component, forming a cage-like structure around aggregated, undegraded proteins at the microtubule organizing center. Vimentin is subject to citrullination under high calcium concentrations, which can occur during macrophage apoptosis, and citrullinated vimentin has been shown to have a role in the production of anti-citrullinated protein antibodies (ACPAs). ACPAs against citrullinated proteins, such as vimentin, are considered to be highly specific markers for rheumatoid arthritis and other autoimmune diseases. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. ELISA: 1:1000. Immunoprecipitation: 5ug/test (20ul resin). Immunofluorescence: 1:100-1:200. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice