Fibronectin (FN, Cold-insoluble Globulin, CIG, FN1) (PE)

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£825.00

SKU:: 058634
Anwendung:: Flow Cytometry
Reinheit:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Purified, human plasma-derived Fibronectin.
Klon:: P1H11
Format:: Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Weitere Details:: Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state, but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels up to 1x10e6 cells. For intracellular staining, cells must first be fixed with 4% paraformaldehyde buffer and permeabilized with 0.1% saponin saline buffer. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Harvest cells and wash twice in saline buffer. 2. Resuspend up to 1x10e6 cells in 0.5ml of cold 4% paraformldehyde fixation buffer and incubate at RT for 10 mins. 3. Following fixation, wash the cells twice in saline buffer, then once in 0.1% saponin saline buffer. 4. After permeabilization, add 10ul of 058634 and incubate the cells for 30 minutes at RT in the dark. 5. Wash the cells twice with 0.1% saponin saline buffer. 6. Resuspend the cells in saline buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with PE-labeled mouse IgG1 antibody. Excitation: 488nm Emission: 565-605nm Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Versandbedingungen:: Blue Ice