Collagen XIII alpha 1 (COL13A1, Collagen 13-alpha 1)

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£832.00

SKU:: 058623
Anwendung:: IHC-Fr
Reinheit:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Glu109-Leu668 from human Collagen XIII alpha 1, expressed in CHO cells.
Klon:: 26A2
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Weitere Details:: COL13A1 (collagen 13-alpha 1) is a 95kD member of the transmembrane group of the collagen family of proteins. It is a type II transmembrane glycoprotein that is expressed by multiple cell types, including fibroblasts, endothelial cells, and cardiac muscle. COL13A1 forms disulfide-linked homotrimers and participates in cell adhesion by binding to the Integrin alpha 1 subunit, Nidogen-2, and Fibronectin. Human COL13A1 is 717 amino acids (aa) in length. It has an N‑terminal 44 aa cytoplasmic region plus a 656 aa extracellular domain (ECD) (aa 62-717). The ECD contains four non-collagenous (NC) regions (aa 1‑121, aa 217-269, aa 442-463, and aa 700-717) interspersed among three collagenous (COL) domains (aa 122-216, aa 270-441, and aa 464-699). Multiple splice forms exist and typically involve deletions of 12-30 aa between aa 220-705. Proteolytic cleavage after Arg108 generates an 85-90kD soluble form. Over aa 109-668 (based on a human isoform [NP_542992} that shows a deletion of aa 239-260, aa 551-565, and aa 616-627), human COL13A1 shares 91% aa sequence identity with mouse COL13A1. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry 8-25ug/ml in immersion fixed paraffin-embedded sections of human placenta as a sample. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice