Bone Morphogenic Protein 6 (BMP6, BMP-6, vgr-1, Vegetal Related, DVR-6, Decapentaplegic Vegetal Related, VGR, VGR1)

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£895.00

SKU:: 058612
Reinheit:: Purified by Protein A affinity chromatography from ascites. Endotoxin: ≤0.10EU/ug (LAL).
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Arg296-His428 from human Bone Morphogenic Protein 6, expressed in CHO cells.
Klon:: 25H37
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Weitere Details:: Human BMP-6 is one of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human and mouse BMP-6 shares 96% amino acid sequence identity. BMP-6 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. Biologically active BMP-6 is a disulfide-linked homodimer of the carboxy-terminal 132 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-6 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. Based on amino acid sequence similarity, BMP-5, -6, -7, and -8 are in the same subgroup. Applications: Suitable for use in ELISA, Neutralization and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (capture): 2-8ug/ml using 058613 as detection antibody. Western Blot: 1ug/ml with recombinant human BMP‑6 under non-reducing conditions only. Neutralization: Measured by its ability to neutralize BMP‑6-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The Neutralization Dose (ND50) is typically 0.5-2.0ug/ml in the presence of 300ng/ml recombinant human BMP‑6. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice