052904X

Calprotectin (L1 Protein, S100A9)

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£767.00

SKU:: 052904X
Anwendung:: IHC-Fr
Reinheit:: Purified by Protein A affinity chromatography
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Affinity purified monocyte membrane preparation. Localization: Cytoplasmic
Klon:: MAC3157R (recomb)
Format:: Supplied as a liquid in PBS. No preservative added. For antibody with sodium azide and BSA, see 052904.
Weitere Details:: L1 or Calprotectin molecule, is an intra-cytoplasmic antigen comprising of a 12kD alpha chain and a 14kD beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. 052904X reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 1-2ug/ml. Prior to staining paraffin sections, product requires HIER: boil tissue sections in 10mM Tris, pH 9.0, 1mM EDTA for 20 min and allow to cool before testing. Optimal working dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice