PL Scramblase 1 (Phospholipid Scramblase 1, PL Scramblase 1, Ca(2+)-dependent Phospholipid Scramblase 1, Erythrocyte Phospholipid Scramblase, MmTRA1b)

Größe

£704.00

SKU:: 052886
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human PL Scramblase 1.
Klon:: 1A8
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Weitere Details:: Phospholipid scramblase 1 (UniProt O15162; also known as Ca(2+)-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase, MmTRA1b, PL scramblase 1) is encoded by the PLSCR1 (also known as MMTRA1B) gene (Gene ID 5359) in human. Plasma membrane phospholipids are distributed asymmetrically between the inner and outer leaflets. Such asymmetrical distribution collapses in response to blood coagulation and apoptosis, resulting in phospholipid “scrambling” between the two leaflets of the plasma membrane. Flippases, floppases, and scramblases are three types enzymes known to mediate transbilayer lipid motion. Flippases and floppases function via ATP-dependent mechanism, while scramblases mediate transbilayer movement in a non-selective and energy-independent manner. Originally identified in 1996 as a 37kD erythrocyte type II transmembrane protein that mediates calcium-dependent membrane phospholipids redistribution, PL Scramblase 1 is the protein product encoded by the founding member of the PLSCR family of genes (PLSCR1-5). All PLSCR family members, with the exception of PLSCR2, possess a proline-rich N-terminal region containing PxxP and PPxY domains, a cysteine-rich region, a conserved calcium-binding domain (EF-hand-like), and a putative transmembrane region enriched in hydrophobic amino acids. In addition, PLSCR1 contains a nuclear localization signal (NLS) and a DNA-binding domain that are essential for its nuclear localization and associated nuclear function. In addition to PLSCRs, TMEM16 and XKR family members have also been reported to mediate scramblase activity. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml detected PL Scramblase 1 in 10ug of A549 cell lysate. Immunohistochemistry (FFPE): 1:50 detected PL Scramblase 1 in mouse colon tissue. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice