APN (CD13, AP-N, hAPN, Alanyl Aminopeptidase, Aminopeptidase M, AP-M, Microsomal Aminopeptidase, Myeloid Plasma Membrane Glycoprotein CD13, gp150)

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£754.00

SKU:: 052870
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Crude cell membrane fraction from adult human papillary and reticular dermal fibroblasts.
Klon:: DF-5
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Weitere Details:: Aminopeptidase N (UniProt: P15144; also known as EC:3.4.11.2, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, AP-M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, CD13) is encoded by the ANPEP (also known as APN, CD13, PEPN) gene (Gene ID: 290) in human. Aminopeptidase N is a single-pass type II membrane protein that is also found as a soluble form in cells. It is widely expressed as a homodimer of 280kD on the cell surface in many tissues, including intestinal epithelia and the nervous system. It serves as a broad specificity aminopeptidase that plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. It preferentially cleaves N-terminus neutral amino acids, most notably alanine residue. Aminopeptidase N is involved in many physiological processes, including antigen presentation regulation, differentiation, proliferation, apoptosis, cancer metastasis, and angiogenesis. It is also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Human Aminopeptidase N consists of a short N-terminal cytoplasmic end (aa2-8), a transmembrane domain (aa9-32), and a large extracellular portion (aa33-967), which is composed of a Ser/Thr-rich region (aa33-68) and the metalloprotease domain (aa69-967). Applications: Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry, Immunofluorescence and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 1:50 detected APN/CD13 in PC3 cell line. Immunohistochemistry (paraffin): 1:50 detected APN/CD13 in human prostate tissue. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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