MMP-2, Catalytic Domain (Gelatinase A, 72 kDa Type IV Collagenase)

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£831.00

SKU:: 052809
Reinheit:: Purified
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: E. coli expressed active rat 72kD type IV collagenase (catalytic domain).
Format:: Supplied as a liquid in PBS, 0.05% sodium azide.
Weitere Details:: MMP-2 [Gelatinase-A] is constitutively produced in quiescent cells and tissues, and the enzyme has a high specific activity against denatured collagen. The low protein levels produced in cell culture (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, 1992) or immunoprecipitation. This can prevent appearance of a spurious band of antibody binding in the vicinity of 68kD displayed by some concentrated media. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:2000 Immunoprecipitation: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.