052784
Nitrotyrosine
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£837.00
- SKU:
- 052784
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Nitrated Keyhole Limpet Hemocyanin (KLH)
- Klon:
- 2A8.2
- Format:
- Supplied as liquid in buffer with 0.09% sodium azide.
- Weitere Details:
- The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 1:50-1:100, detects nitrated proteins in formalin-fixed paraffin embedded mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Western Blot: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice