Butyrylcholinesterase, Recombinant, Human, aa29-602, His-Tag (BCHE)

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£881.00

SKU:: 052759
Molekulargewicht:: 68.4
Reinheit:: ≥95% (SDS-PAGE)
Lagerbedingungen:: -70°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in in 50mM HEPES, pH 7.6, 150mM sodium chloride.
Weitere Details:: Butyrylcholinesterase (BChE) is a serine hydrolase that preferentially hydrolyzes butyrylcholine (BCh) but can also hydrolyze acetylcholine (ACh).1 It is composed of a large catalytic domain and C-terminal tetramerization domain.2 BChE can exist as a monomer but primarily oligomerizes into soluble or membrane-anchored tetramers by combining with polyproline-rich peptides, such as lamellipodin.3,2 It is widely expressed with high levels in the plasma, liver, lungs, and brain.4,1 In addition to its hydrolysis of BCh and ACh, BChE also hydrolyzes certain ester-containing exogenous substances.5 Due to this, loss-of-function mutations in BCHE are associated with the accumulation of exogenous BChE substrates, including the muscle relaxant succinylcholine, which can lead to adverse effects such as prolonged apnea.5,6 BChE is implicated in the regulation of the peptide hormone ghrelin, and dysregulation of BChE is found in various conditions, including Alzheimer’s disease, where it is found in amyloid-β plaques and neurofibrillary tangles, and sudden infant death syndrome (SIDS), where it is found at reduced levels in infant dried blood spots.7,1,8 052759 can be used for enzyme activity assays. Active recombinant protein corresponding to aa29-602 frin human BChE, C-terminal His-Tagged, expressed in mammalian cells. Swiss Prot: P06276 Molecular Weight: ~68.4kD Specific Activity: As Reported Unit Definition: One unit is defined as the amount of enzyme required to produce 1umol of thiocholine/minute at -23°C in PBS, pH 7.4 containing 1mM acetylthiocholine as substrate. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Dry Ice